Research Divisions

Electroanalysis

Group LeaderLocation and ContactMembersLines of ResearchNotable PublicationsProjectsPhD ThesesClinical TrialsPatents and TrademarksNews
Prof. María Jesús Lobo Castañón ISPA Research Groups Electroanalysis
Professor María Jesús Lobo Castañón

María Jesús Lobo Castañón completed her PhD in Chemistry at the Universidad de Oviedo in July 1996, for which she received an Outstanding PhD Thesis Award. She has been lead researcher of the Electroanalysis research group (Universidad de Oviedo) since October 2011, and Professor of Analytical Chemistry in the Department of Physical and Analytical Chemistry of the Universidad de Oviedo since January 2017: a position in which she combines teaching on the Bachelor of Chemistry, the Bachelor of Biotechnology and Master of Analytical and Bioanalytical Sciences with research, development and innovation activities.

Her research focuses on the development of electrochemical sensors (amperometric and voltammetric sensors, as well as those based on impedance spectroscopy) in which the recognition elements used are natural receptors such as enzymes and nucleic acids, and others that mimic natural receptors, such as aptamers. She also has experience using surface plasmon resonance spectroscopy for characterising affinity interactions and designing sensors, After her PhD training period, under the supervision of Professors Paulino Tuñón Blanco and Arturo J. Miranda Ordieres, she completed postdoctoral research at two prestigious international research centres. The first fellowship was at the Department of Pure and Applied Biochemistry of Lund University in Sweden, as part of the research group coordinated by Professor Klaus Mosbach, a pioneer of the design and synthesis of molecularly imprinted polymers. The second was at the Nanobioelectronics Laboratory of the Department of Nanoengineering at the University of California San Diego, led by Professor Joseph Wang, one of the most internationally influential scientists in the electrochemistry field.

She is currently principal investigator on a research project, secured through a competitive national funding round, which focuses on detecting proteins that have potential as tumour markers, using electrochemical sensors.

She participated in European project FP7- PEOPLE-2013-IRSES, “Monitoring genetically modified organisms in food and feed by innovative biosensor approaches”, as coordinator of the Universidad de Oviedo team.

She is author of one patent, five book chapters and over 100 research articles, with a H-index of 29. She has supervised nine PhD theses and a large number of Master’s and Bachelor’s dissertations in Biotechnology. She has ongoing partnerships with prestigious research groups in the sensor-development field in Italy, Portugal, Germany, Hungary, the United States and France.

She is a member of the International Society of Electrochemistry (ISE), the Sociedad Española de Química Analítica (Spanish Society of Analytical Chemistry) and the American Chemical Society (ACS). She is also a member of the Sensors editorial board and the advisory board of Analytical and Bioanalytical Chemistry.

She participates as a reviewer in specialised scientific journals in the electrochemistry and analytical chemistry field, such as Analytical Chemistry, Trends in Analytical Chemistry, Analytica Chimica Acta, Talanta, Analytical and Bioanalytical Chemistry, Biosensors & Bioelectronics, Sensors and Actuators B, Electroanalysis and Journal of Electroanalytical Chemistry.

Departamento de Química Física y Analítica

Facultad de Química. Universidad de Oviedo

Avenida Julián Clavería 8. 33006 OVIEDO, Spain

Name Organisation Activity Euraxess classification
Abardía Serrano, Clara Universidad de Oviedo Research R1
Astobieta Madariaga, Elene Universidad de Oviedo Research R1
de los Santos Álvarez, Noemí Universidad de Oviedo Research/Teaching R3
Gómez Meijide, Paula Universidad de Oviedo Research R1
Lobo Castañón, María Jesús Universidad de Oviedo Research/Teaching R4
Miranda Castro, Rebeca Universidad de Oviedo Research/Teaching R2
Sánchez Salcedo, Raquel Universidad de Oviedo Research R1
Stefan , Geanina Maria Universidad de Oviedo Research R1

ISPA staff in the Electroanalysis research group

History of the Group

The Electroanalysis research group has been developing electrochemical biosensors for over 30 years, working continuously on 15 research projects financed through competitive public funding. We are recognised as a consolidated research group by the Principado de Asturias and the Universidad de Oviedo. Our current priority is the development of chemical sensors based on the use of nucleic acids as a molecular recognition element, either with aptamers or by using the hybridisation reaction, in order to solve significant problems in the biomedical and food sectors.
One of the challenges we have set ourselves is the selection of aptamers against proteins identified as having potential as tumour biomarkers. The aptamers obtained may be used not only to develop chemical sensors, but also in preconcentration procedures for circulating proteins, or even in the development of therapeutic antitumour agents.

  • Selection of aptamers for the recognition of proteins that act as tumour biomarkers and the development of electrochemical sensors based on those receptors.
    ISPA Electroanalysis Research Group Line of Research 1
    Process of in vitro selection, known as SELEX, for obtaining aptamers against the protein glycosylation site (Anal. Bioanal. Chem. 410 (2018) 2059-2065)
  • Development of DNA devices for the detection of pathogenic organisms, based on integrating the methods of isothermal amplification of nucleic acids and electrochemical detection.
    ISPA Electroanalysis research group Line of Research 2
    A platform upon surfaces of indium and tin oxides (ITO), of general application for quantifying genomic DNA, based on helicase-dependent isothermal amplification with electrochemical detection. Chem. Commun 53 (2017) 9721-9724.
  1. AUTHORS: Alexandra Plácido, Clara Pereira, Alexandra Guedes, M. Fátima Barroso, Rebeca Miranda-Castro, Noemí de-los-Santos-Álvarez, Cristina Delerue-Matos
    TITLE: Electrochemical genoassays on gold-coated magnetic nanoparticles to quantify genetically modified organisms (GMOs) in food and feed as GMO percentage
    JOURNAL: Biosensors and Bioelectronics 110, 147-154, 2018. IF=8.173 Q1 (2/80) Chemistry, Analytical.
  2. AUTHORS: Susana Barreda-García, Rebeca Miranda-Castro, Noemí de-los-Santos-Álvarez, Arturo J. Miranda-Ordieres, María Jesús Lobo-Castañón.
    TITLE: Sequence-specific electrochemical detection of enzymatic amplification products of Salmonella genome on ITO electrodes improves pathogen detection to the single copy level
    JOURNAL: Sensors and Actuators B 268, 438-445, 2018. IF=5.667 Q1 (7/80) Chemistry, Analytical.
  3. AUTHORS: Ana Díaz-Fernández, Rebeca Miranda-Castro, Noemí de-los-Santos-Álvarez, María Jesús Lobo-Castañón.
    TITLE: Post-translational modifications in tumor biomarkers: the next challenge for aptamers?
    JOURNAL: Analytical and Bioanalytical Chemistry 410(8), 2059-2065, 2018. IF=3.307 Q1 (15/80) Chemistry, Analytical.
  4. AUTHORS: Susana Barreda-García, Rebeca Miranda-Castro, Noemí de-los-Santos-Álvarez, Arturo J. Miranda-Ordieres, María Jesús Lobo-Castañón
    TITLE: Helicase-dependent isothermal amplification: a novel tool in the development of molecular-based analytical systems for rapid pathogen detection
    JOURNAL: Analytical and Bioanalytical Chemistry 410, 679-693, 2018. IF=3.307 Q1 (15/80) Chemistry, Analytical.
  5. AUTHORS: Susana Barreda-García, Rebeca Miranda-Castro, Noemí de-los-Santos-Álvarez, Arturo J. Miranda-Ordieres, María Jesús Lobo-Castañón
    TITLE: Solid-phase helicase dependent amplification and electrochemical detection of Salmonella on highly stable oligonucleotide-modified ITO electrodes.
    JOURNAL: Chemical Communications 53, 9721-9724, 2017. IF=6.290 Q1 (28/171) Chemistry, Multidisciplinary.
  6. AUTHORS: Laura López-López, Rebeca Miranda-Castro, Noemí de-los-Santos-Álvarez, Arturo J. Miranda-Ordieres, María Jesús Lobo-Castañón
    TITLE: Disposable electrochemical aptasensor for gluten determination in food
    JOURNAL: Sensors and Actuators B 241, 522-527, 2017.
    IF=5.667 Q1 (7/80) Chemistry, Analytical.
  7. AUTHORS: Rebeca Miranda-Castro, Raquel Sánchez-Salcedo, Beatriz Suárez-Álvarez, Noemí de-los-Santos-Álvarez, Arturo J. Miranda-Ordieres, María Jesús Lobo-Castañón
    TITLE: hioaromatic DNA monolayers for target-amplification-free electrochemical sensing of environmental pathogenic bacteria.
    JOURNAL: Biosensors and Bioelectronics 92, 162-170, 2017. IF=8.173 Q1 (2/80) Chemistry, Analytical.
  8. AUTHORS: Begoña Martín-Fernández, Noemí de-los-Santos-Álvarez, Juan Pedro Martín- Clemente, María Jesús Lobo-Castañón, Beatriz López-Ruiz
    TITLE: Challenging genosensors in food samples: the case of gluten determination in highly processed samples.
    JOURNAL: Talanta 146, 490-496, 2016.
    IF=4.162 Q1 (9/76) Chemistry, Analytical.
  9. AUTHORS: M. Freitas, Maria Sá Couto, M.F. Barroso, Clara Pereira, N. de-los-Santos- Álvarez, A. J. Miranda-Ordieres, M.J. Lobo-Castañón, C. Delerue-Matos
    TITLE: Highly monodisperse Fe3O4@Au superparamagnetic nanoparticles as reproducible platform for genosensing genetically modified organisms.
    JOURNAL: ACS Sensors 1, 1044-1053, 2016.
    IF=5.711 Q1 (33/171) Chemistry, Multidisciplinary.
  10. AUTHORS: Susana Barreda-García, Rebeca Miranda-Castro, Noemí de-los-Santos-Álvarez, Arturo J. Miranda-Ordieres, María Jesús Lobo-Castañón
    TITLE: Comparison of isothermal helicase dependent amplification and PCR for the detection of Mycobacterium tuberculosis by an electrochemical genomagnetic assay.
    JOURNAL: Analytical and Bioanalytical Chemistry 408(30), 8603-8610, 2016.
    IF=3.431 Q1 (16/76) Chemistry, Analytical.
  11. AUTHORS: Suely Moura-Melo, Rebeca Miranda-Castro, Noemí de-los-Santos-Álvarez, Arturo J. Miranda-Ordieres, José Ribeiro Dos Santos Júnior, Rosana A. da Silva-Fonseca, María Jesús Lobo-Castañón
    TITLE: Targeting Helicase-Dependent Amplification Products with an Electrochemical Genosensor for Reliable and Sensitive Screening of Genetically Modified Organisms.
    JOURNAL: Analytical Chemistry 87(16), 8547-8554, 2015. IF=5.886 Q1 (4/75) Chemistry, Analytical.
  12. AUTHORS: Sonia Amaya-González, Laura López-López, Rebeca Miranda-Castro, Noemí de- los-Santos-Álvarez, Arturo J. Miranda-Ordieres, María Jesús Lobo-Castañón.
    TITLE: AFFINITY OF APTAMERS BINDING 33-MER GLIADIN PEPTIDE AND GLUTEN PROTEINS: INFLUENCE OF IMMOBILIZATION AND LABELING TAGS
    JOURNAL: Analytica Chimica Acta 873, 63-70, 2015. IF=4.712 Q1 (8/75) Chemistry, Analytical.
  13. AUTHORS: M. Fátima Barroso, María Freitas, M. Beatriz P.P. Oliveira, Noemí de-los-Santos- Álvarez, María Jesús Lobo-Castañón, Cristina Delerue-Matos.
    TITLE: 3D-nanostructured Au electrodes for the event-specific detection of MON810 transgenic maize.
    JOURNAL: Talanta 134, 158-164, 2015.
    IF=4.035 Q1 (9/75) Chemistry, Analytical.
  14. AUTHORS: S. Barreda-García, M.J. González-Álvarez, N. de-los-Santos-Álvarez, A. J. Miranda-Ordieres, M. J. Lobo-Castañón
    TITLE: Attomolar quantitation of Mycobacterium tuberculosis by asymmetric helicase-dependent isothermal DNA-amplification and electrochemical detection.
    JOURNAL: Biosens. Bioelectron. 68, 122-128, 2015. IF=7.476 Q1 (3/75) Chemistry, Analytical.
  15. AUTHORS: C. Lorena Manzanares-Palenzuela, Noemí de-los-Santos-Álvarez, María Jesús Lobo-Castañón, Beatriz López-Ruiz.
    TITLE: Multiplex electrochemical DNA platform for femtomolar-level quantification of genetically modified soybean.
    JOURNAL: Biosens. Bioelectron. 68, 259-265, 2015. IF=7.476 Q1 (3/75) Chemistry, Analytical.
  16. AUTHORS: S. Amaya-González, Noemí de-los-Santos-Álvarez, A.J. Miranda-Ordieres, María Jesús Lobo-Castañón.
    TITLE: Sensitive Gluten Determination in Gluten Free Foods by an Electrochemical Aptamer-based Assay.
    JOURNAL: Analytical Bioanalytical Chemistry 407, 6021-6029, 2015. IF=3.125 Q1 (15/75) Chemistry, Analytical.
  17. AUTHORS: Sonia Amaya-González, Noemí de-los-Santos-Álvarez, Arturo J. Miranda- Ordieres, María Jesús Lobo-Castañón
    TITLE: Aptamer binding to celiac disease-triggering hydrophobic proteins: a sensitive gluten detection approach.
    JOURNAL: Analytical Chemistry 86(5), 2733-2739, 2014. IF=5.636 Q1 (4/74) Chemistry, Analytical.
  18. AUTHORS: B. Martín-Fernández, A.J. Miranda-Ordieres, M.J. Lobo-Castañón, G. Frutos- Cabanillas, N. de-los-Santos-Álvarez, B. López-Ruiz
    TITLE: Strongly structured DNA sequences as targets for genosensing: sensing phase design and coupling to PCR amplification for a highly specific 33-mer gliladin DNA frament.
    JOURNAL: Biosens. Bioelectron. 60, 244-251, 2014.
    IF=6.409 Q1 (3/74) Chemistry, Analytical.
  19. AUTHORS: Marta Sánchez-Paniagua López, Gloria Frutos Cabanillas, M. Jesús Lobo Castañón, Beatriz López-Ruiz.
    TITLE: Development of a genosensor for peanut allergen ARA h 2 detection and its optimization by surface response methodology.
    JOURNAL: Biosens. Bioelectron. 62, 350-356, 2014. IF=6.409 Q1 (3/74) Chemistry, Analytical.
  20. AUTHORS: E. González-Fernández, N. de-los-Santos-Álvarez, A. J. Miranda-Ordieres, M. J. Lobo-Castañón
    TITLE: Monovalent labeling system improves the sensitivity of aptamer-based inhibition assay for small molecule detection.
    JOURNAL: Sensors and Actuators: B. Chemical 182, 668-674, 2013. IF=3.840 Q1 (8/76) Chemistry, Analytical.
Principal Investigator Title Funding Body Duration Amount
María Jesús
Lobo Castañón
Noemí de-los-Santos-Álvarez
Selection and Modification of aptamers as specific receptors for the detection of serum tumour biomarkers (MINECO-16-CTQ2015-63567- R) Ministerio de Economía y Competitividad 01-01-2016 to 31-12-2018 €89,540
María Jesús
Lobo Castañón
Food Bioanalysis Group (FC-15-GRUUPIN14-025) Consejería de Economía y Empleo de Asturias 31-12-2014 to 31-12-2017 €126,400
María Jesús
Lobo Castañón
Monitoring Genetically Modified Organisms in food and feed through innovative biosensor approaches (UE-13-GMOSENSOR-612545) European Union 01-10-2013 to 30-09-2015 €327,600
María Jesús
Lobo Castañón
Detection of allergenic proteins and pathogenic agents in food using electrochemical sensors (MINECO-13-CTQ 2012-31157) Ministerio de Economía y Competitividad 01-01-2013 to 31-12-2015 €99,450
PhD Student Supervisors Title Year Honours
Susana Barreda García M.J. Lobo Castañón
N. de los Santos Álvarez
Electrochemical devices for the detection of pathogens based on helicase dependent isothermal DNA-amplification 2017 No
Carmen Lorena Manzanares Palenzuela M.J. Lobo Castañón
B. López Ruiz
Electrochemical DNA biosensors and sensing platforms for the detection and quantification of genetically modified soybean in food and feed 2017 International
Suely Moura Melo J. Ribeiro dos Santos
R. Miranda Castro
An electrochemical sensor for detecting genetically-modified organisms: detection of the P35S promoter 2016 No
Daniel Antuña Jiménez A.J. Miranda Ordieres
M.C. Blanco López
Development of sensing phases for the detection of tumour markers with electrochemical and optical sensors 2015 European
Begoña Martín Fernández B. López Ruiz
N. de los Santos Álvarez
Indirect identification of gluten using methods based on DNA detection: PCR and electrochemical genosensors 2015 European
Sonia Amaya González M.J. Lobo Castañón
N. de los Santos Álvarez
Selection of aptamers against allergenic gluten proteins and their application in electrochemical trials 2015 European
Eva González Fernández M.J. Lobo Castañón
N. de los Santos Álvarez
Structured nucleic acids as affinity receptors for designing electrochemical sensors 2012 International
Inventors Title Registration No
N. de-los-Santos-Álvarez, S. Amaya-González,
A.J. Miranda-Ordieres, M.J. Lobo-Castañón
Specific aptamers against gluten and a detection method for the associated gluten ES2436861 B2